Maintaining compliance with industry standards and regulations is crucial in the healthcare sector and food manufacturing environments. SESA’s Mould, Yeast & Bacteria Air Testing and Surface Testing services are designed to help you meet these requirements. Our Occupational Hygienists are well-versed in the guidelines and standards set forth by regulatory bodies. We conduct our testing in accordance with these standards, ensuring that your facility meets the necessary criteria for patient safety and infection control and/or surface cleanliness and non-contamination. With SESA as your partner, you can have confidence in the cleanliness and safety of your operating theatres, healthcare and other critical environments.
The microorganisms sampling will include sampling airborne bacteria, mould and yeast in the operating theatre and if required in adjacent rooms (eg. sterile stock room). Duplicate samples will be collected for airborne bacteria and duplicate samples will be collected for mould and yeast in each room. The average of the two duplicates will be used for comparison with the guidelines.
Prior to testing the following requirements must be met:
- The rooms shall be empty of all non-fixed items, including supplies, sterile stock and any mobile equipment.
- No microbiological air sampling shall be conducted until:
- all building and construction works have been completed
- all HVAC commissioning procedures have been completed
- all the ducting and air diffuser plates have been cleaned
- the room being sampled has been thoroughly cleaned, including ceiling spaces, service cavities, ventilation grills, all horizontal and vertical
- surfaces including ceilings and walls, and any fixed equipment in the room
- a second clean of all horizontal and vertical surfaces within the room is recommended to ensure any contamination is removed
- the HVAC system has been running continuously, on normal flow rates, for at least 24 hours following completion of all building and construction. Cleaning can be performed during this period of time
- No construction workers or non-sterile materials to enter the room after the cleaning
The samples will be aseptically handled as required and transported under a chain of custody to NATA accredited laboratory for analysis in accordance with their NATA accredited method which involves an incubation period of 3 days for bacteria samples and 5 days for fungi (mould and yeast) samples.
OD: 0610/15 states that “Aerobic cultures on non-selective media should not exceed 10 bacterial and or fungal CFUs per cubic metre (m3) of air sampled”. This criterion will be used to evaluate the analytical results of the aerobic culture samples.